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Öğe An Electronic Control and Image Acquisition System for Laser Scanning Microscopy(IEEE, 2015) Gumus, Gokhan; Sarioglu, Baykal; Gokdel, Yigit DaghanThis paper presents an electronic system that controls the entire operation of a laser scanning microscopy system through a DAQ card. Proposed system does not only create the required electro-coil driving signal peculiar to magnetically actuated micro-scanner that enables the raster-scanning movement, but also is responsible from the image acquisition part by both serially gathering the laser intensity data and using it to construct a meaningful microscopy image. Micro-scanner which is fabricated using Ni as the structural material is utilized in the system. The microscanner's slow and fast scan frequencies are measured to be 250 Hz and 1560 Hz, respectively. Model of the DAQ card used in the system is NI-6356 which has maximum 5 mA current and 10 V voltage outputs. A power amplifier circuit with LM 386 is designed and added to the system for increasing field-of-view of the micro-scanner. The operation of the proposed system is demonstrated by acquiring data and constructing images from the USAF resolution target.Öğe System Integration for Real-Time Laser Scanning Confocal Microscope(IEEE, 2016) Gumus, Gokhan; Sarioglu, Baykal; Gokdel, Yigit DaghanIn this work, a laser scanning confocal microscopy system governed by a software controlled DAQ Card is presented. The presented system can be utilized for scanning a target and displaying the resulting image through a designed graphical user interface (GUI). The system performs two main operations: (1) generation of the actuation signal and (2) image acquisition. The architecture of the proposed system and successful operation of the system is demonstrated by constructing images from USAF51 negative resolution test target. In the experiments, the proposed system is operated at the slow scan frequency (f (s)) of 1 Hz and the fast scan frequency (f (fast)) of 100 Hz with a sampling frequency (f (fast)) of 20 kHz. The experimental results show that 1 mu m lateral resolution is achieved in the proposed system.