Identification of a novel gene set in human cumulus cells predictive of an oocyte's pregnancy potential

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dc.authoridJ. M. Rosa, Guilherme/0000-0001-9172-6461|Crosby, Javier A./0009-0001-8504-754X|Cibelli, Jose/0000-0002-2176-3854|Otu, Hasan/0000-0002-9253-8152
dc.authorwosidJ. M. Rosa, Guilherme/G-3862-2011
dc.contributor.authorIager, Amy E.
dc.contributor.authorKocabas, Arif M.
dc.contributor.authorOtu, Hasan H.
dc.contributor.authorRuppel, Patricia
dc.contributor.authorLangerveld, Anna
dc.contributor.authorSchnarr, Patricia
dc.contributor.authorSuarez, Mariluz
dc.date.accessioned2024-07-18T20:42:38Z
dc.date.available2024-07-18T20:42:38Z
dc.date.issued2013
dc.departmentİstanbul Bilgi Üniversitesien_US
dc.description.abstractObjective: To identify a gene expression signature in human cumulus cells (CCs) predictive of pregnancy outcome across multiple clinics, taking into account the clinic and patient variations inherent in IVF practice. Design: Retrospective analysis of single human cumulus-oocyte complexes with the use of a combined microarray and quantitative reverse-transcription polymerase chain reaction (qRT-PCR) approach. Setting: Multiple private IVF clinics. Patient(s): Fifty-eight patients. Samples from 55 patients underwent qRT-PCR analysis, and samples from 27 patients resulted in live birth. Intervention(s): Gene expression analysis for correlation with pregnancy outcome on individual human CCs collected immediately after oocyte retrieval. Pregnancy prediction analysis used leave-one-out cross-validation with weighted voting. Main Outcome Measure(s): Combinatorial expression of 12 genes in 101 samples from 58 patients. Result(s): We found a set of 12 genes predictive of pregnancy outcome based on their expression levels in CCs. This pregnancy prediction model had an accuracy of 78%, a sensitivity of 72%, a specificity of 84%, a positive predictive value of 81%, and a negative predictive value of 76%. Receiver operating characteristic analysis found an area under the curve of 0.763 +/- 0.079, significantly greater than 0.5 (random chance prediction). Conclusion(s): Gene expression analysis in human CCs should be considered in identifying oocytes with a high potential to lead to pregnancy in IVF-ET. (Fertil Steril (R) 2013;99:745-52. (C) 2013 by American Society for Reproductive Medicine.)en_US
dc.description.sponsorshipGema Diagnosticsen_US
dc.description.sponsorshipSupported by Gema Diagnostics.en_US
dc.identifier.doi10.1016/j.fertnstert.2012.10.041
dc.identifier.endpage+en_US
dc.identifier.issn0015-0282
dc.identifier.issn1556-5653
dc.identifier.issue3en_US
dc.identifier.pmid23200692en_US
dc.identifier.scopus2-s2.0-84875230255en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage745en_US
dc.identifier.urihttps://doi.org/10.1016/j.fertnstert.2012.10.041
dc.identifier.urihttps://hdl.handle.net/11411/7366
dc.identifier.volume99en_US
dc.identifier.wosWOS:000315589300026en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofFertility and Sterilityen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectHuman Cumulus Cellsen_US
dc.subjectGene Expressionen_US
dc.subjectPregnancy Outcomeen_US
dc.subjectHuman Oocyteen_US
dc.subjectHuman Embryoen_US
dc.subjectExpression Profilesen_US
dc.subjectEmbryo Competenceen_US
dc.subjectMarker Genesen_US
dc.subjectFertilizationen_US
dc.subjectIndicatoren_US
dc.subjectAssociationen_US
dc.subjectMetabolismen_US
dc.subjectReceptoren_US
dc.subjectCanceren_US
dc.titleIdentification of a novel gene set in human cumulus cells predictive of an oocyte's pregnancy potentialen_US
dc.typeArticleen_US

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