Gene expression profiling of granulosa cells from PCOS patients following varying doses of human chorionic gonadotropin

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dc.WoS.categoriesGenetics & Heredity; Obstetrics & Gynecology; Reproductive Biologyen_US
dc.authorid0000-0002-9253-8152en_US
dc.contributor.authorOtu, Hasan H.
dc.date.accessioned2021-02-24T13:01:03Z
dc.date.available2021-02-24T13:01:03Z
dc.date.issued2013-03
dc.description.abstractHuman chorionic gonadotrophin (hCG) has been used to induce ovulation and oocyte maturation. Although the most common dose of hCG used in IVF is 10,000 IU, there are reports that suggest 5,000 IU is sufficient to yield similar results. The objective of this study is to evaluate the dose dependent differences in gene expression of granulosa cells following various doses of hCG treatment. Patients with polycystic ovarian syndrome (PCOS) were stimulated for IVF treatment. The hCG injection was either withheld or given at 5,000 or 10,000 IU. Granulosa cells from the follicular fluids have been collected for RNA isolation and analyzed using Affymetrix genechip arrays. Unsupervised hierarchical clustering based on whole gene expression revealed two distinct groups of patients in this experiment. All untreated patients were clustered together whereas hCG-treated patients separated to a different group regardless of the dose. A large number of the transcripts were similarly up- or down-regulated across both hCG doses (2229 and 1945 transcripts, respectively). However, we observed dose-dependent statistically significant differences in gene expression in only 15 transcripts. Although hCG injection caused a major change in the gene expression profile of granulosa cells, 10,000 IU hCG resulted in minimal changes in the gene expression profiles of granulosa cells as compared with 5,000 IU. Thus, based on our results, we suggest the use of 10,000 IU hCG should be reconsidered in PCOS patients.en_US
dc.fullTextLevelFull Texten_US
dc.identifier.doi10.1007/s10815-013-9935-y
dc.identifier.issn1573-7330
dc.identifier.issn1058-0468
dc.identifier.pmid23381551en_US
dc.identifier.scopus2-s2.0-84876296689en_US
dc.identifier.urihttps://hdl.handle.net/11411/3295
dc.identifier.urihttps://doi.org/10.1007/s10815-013-9935-y
dc.identifier.wosWOS:000316753300007en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.issue3en_US
dc.language.isoenen_US
dc.nationalInternationalen_US
dc.numberofauthors5+en_US
dc.pages341-352en_US
dc.publisherSpringer/Plenum Publishersen_US
dc.relation.ispartofJournal of Assisted Reproduction and Geneticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGranulosa cellsen_US
dc.subjectGene expressionen_US
dc.subjecthCGen_US
dc.subjectMicroarray/PCOSen_US
dc.titleGene expression profiling of granulosa cells from PCOS patients following varying doses of human chorionic gonadotropin
dc.typeArticle
dc.volume30en_US

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